Meet the challenges of this rapidly expanding field. Molecular diagnostics continues to grow in importance in the clinical laboratory. This respected text will prepare your students with a grounding in the fundamental principles of molecular biology. current methods, and their clinical applications. With a focus on the application of molecular concepts to diagnostic purposes, the text explains and illustrate the use and interpretation of molecular-based assays in patient care. Now with an expanded discussion of nucleic acid sequencing, with added emphasis on next generation sequencing (NGS), and updated coverage of proteomics and mass spectrometry applications, your students will have the most current information available today.
I. Fundamentals of Molecular Biology: An Overview 1. Nucleic Acids and Proteins 2. Gene Expression and Epigenetics II. Common Techniques in Molecular Biology 3. Nucleic Acid Extraction Methods 4. Resolution and Detection of Nucleic Acids 5. Analysis and Characterization of Nucleic Acids and Proteins 6. Nucleic Acid Amplification 7. Chromosomal Structure and Chromosomal Mutations 8. Gene Mutations 9. DNA Sequencing III. Techniques in the Clinical Laboratory 10. DNA Polymorphisms and Human Identification 11. Detection and Identification of Microorganisms 12. Molecular Detection of Inherited Diseases 13. Molecular Oncology 14. DNA-Based Tissue Typing 15. Quality Assurance and Quality Control in the Molecular Laboratory Appendices A. Study Questions Answers B. Answers to Case Studies Glossary Index
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