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Neuroscience is, by definition, a multidisciplinary field: some scientists study genes and proteins at the molecular level while others study neural circuitry using electrophysiology and high-resolution optics. A single topic can be studied using techniques from genetics, imaging, biochemistry, or electrophysiology. Therefore, it can be daunting for young scientists or anyone new to neuroscience to learn how to read the primary literature and develop their own experiments. This volume addresses that gap, gathering multidisciplinary knowledge and providing tools for understanding the neuroscience techniques that are essential to the field, and allowing the reader to design experiments in a variety of neuroscience disciplines.
Chapter 1: Whole Brain Imaging Chapter 2: Animal Behavior Chapter 3: Stereotaxic Surgeries and In vivo techniquesChapter 4: ElectrophysiologyChapter 5: MicroscopyChapter 6: Visualizing Nervous System Structure Chapter 7: Visualizing Nervous System FunctionChapter 8: Manipulating Neural Activity Chapter 9: Identifying Genes and Proteins of InterestChapter 10: Molecular Cloning and Recombinant DNA TechnologyChapter 11: Gene Delivery StrategiesChapter 12: Making and using transgenic organismsChapter 13: Manipulating endogenous genes Chapter 14: Cell culture techniquesChapter 15: Biochemical assays and intracellular signaling